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Registros recuperados: 24
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A new methodology for the improvement of diagnostic immunohistochemistry in canine veterinary pathology: automated system using human monoclonal and polyclonal antibodies Arq. Bras. Med. Vet. Zootec.
Cassali,G.D.; Silva,P.; Rêma,A.; Gärtner,F.; Gobbi,H.; Tafuri,W.L.; Schmitt,F.C..
The authors describe their experience with an automated immunohistochemical system applied to canine tissue samples. Twenty human cellular markers specific monoclonal and polyclonal antibodies and two different antigen retrieval methods were used in normal and neoplastic breast tissue, as well as skin samples obtained from female dogs of pure and mixed breeds. The antibodies tested were the most frequently used in human and veterinary medicine studies, employed with diagnostic purposes in breast pathology, as well as in cancer research. Most of them may be used to study other normal and abnormal tissues and included cytokeratins, progesterone receptor, c-erbB2, p53, MIB-1, PCNA, EMA, vimentin, desmin, alpha-actin, S-100, pan-cadherin, and E-cadherin. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Immunohistochemistry; Automated system; Mammary gland.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000300010
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Adiponectin serum levels in female dogs bearing mammary gland carcinomas Arq. Bras. Med. Vet. Zootec.
Souza,I.B.R.; Cardoso,C.V.; Pereira,A.A.F.; Poppe,S.C.; Bondan,E.F..
RESUMO Em seres humanos, a adiponectinemia está associada à obesidade e ao risco aumentado a uma ampla variedade de cânceres. Embora o papel dessa adipocina esteja bem documentado na patogênese do câncer em humanos, tal associação permanece a ser determinada em cães. Nesses animais, a relação da adiponectina com a carcinogênese parece ser ainda meramente especulativa. Nesse contexto, buscou-se nesta investigação comparar os níveis séricos de adiponectina em fêmeas hígidas e em portadoras de carcinomas mamários com diagnóstico histopatológico de carcinoma mamário tubular simples estágio 4, com comprometimento de linfonodos, porém sem metástases a distância detectadas. Foi observado que as cadelas diagnosticadas com carcinoma mamário tiveram níveis séricos...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Dogs; Adiponectin; Cancer; Mammary gland; Obesity.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000501741
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Bubaline mastitis etiology in Northeast of Brazil Arq. Bras. Med. Vet. Zootec.
Medeiros,E.S.; Freitas,M.F.L.; Pinheiro Júnior,J.W.; Saukas,T.N.; Krewer,C.C.; Santos,A.S.; Costa,M.M.; Mota,R.A..
Este estudo teve como objetivo avaliar a frequência de mastite clínica e subclínica e descrever os micro-organismos envolvidos no processo inflamatório da glândula mamária nos rebanhos de búfalos leiteiros no Nordeste do Brasil. Foram analisadas 1.896 amostras de leite provenientes de 474 búfalos em quatro propriedades localizadas nos Estados de Alagoas, Bahia, Ceará e Pernambuco. Após o exame físico da glândula mamária, as amostras de leite de cada teto foram submetidas aos testes da caneca do fundo preto e CMT (California Mastitis Test). As amostras que apresentaram scores ++ e +++ no CMT e as positivas para a caneca do fundo preto foram submetidas ao exame microbiológico. Do total de amostras estudadas, 90/1.896 (4,74%) apresentaram mastite clínica. Com...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Milk; Mammary gland; Buffaloes; Microorganisms.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000600043
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Ca2+-activated Cl- channel currents in mammary secretory cells from lactating mouse OAK
Kamikawa, Akihiro; Ichii, Osamu; Sakazaki, Junpei; Ishikawa, Toru.
The Cl- secretion via Ca2+-activated Cl- channel (CaCC) is critical for fluid secretion in exocrine glands like the salivary gland. Also in the mammary gland, it has been hypothesized that CaCC plays an important role in the secretion of Cl- and aqueous phase of milk. However, there has been no evidence for the functional expression of CaCC in native mammary secretory (MS) cells of lactating animals. We therefore assessed membrane current in MS cells that were freshly isolated from lactating mice using whole cell patch-clamp techniques. In MS cells, we detected CaCC current that exhibited the following characteristics: 1) Ca2+-dependent activation at the concentrations of sub-micromolar range; 2) voltage-dependent activation; 3) slow kinetics for...
Palavras-chave: CaCC; Lactation; Mammary gland; Mammary secretory cell; Patch-clamp.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4553
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Dopaminergic modulation of grooming behavior in virgin and pregnant rats BJMBR
Serafim,A.P.; Felicio,L.F..
Dopamine receptors are involved in the expression of grooming behavior. The pregnancy-induced increase in self-licking observed in rats is important for mammary gland development and lactation. This study focuses on the role of dopamine receptor subtypes in grooming behavior of virgin and pregnant female rats. General and mammary gland grooming were measured in virgin rats treated with 0.25 mg/kg of the D1-like agonist SKF-81297 and antagonist SKF-83566 and the D2-like agonist lisuride and antagonist sulpiride. The effects of 0.01 and 0.25 mg/kg doses of the same agonists and antagonists were evaluated in pregnant rats as well. In virgin animals both SKF-83566 and sulpiride treatments significantly reduced the time spent in general grooming, while none of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Self-licking; Lisuride; SKF-81297; SKF-83566; Sulpiride; Mammary gland; Prolactin; Dopamine.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100015
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Epidemiological, clinical and pathological evaluation of overall survival in canines with mammary neoplasms Arq. Bras. Med. Vet. Zootec.
Nunes,F.C.; Campos,C.B.; Teixeira,S.V.; Bertagnolli,A.C.; Lavalle,G.E.; Cassali,G.D..
ABSTRACT Canine mammary neoplasms (CMNs) are the most frequent lesions and in female dogs. However, studies correlating pathological criteria with clinical evolution in female dogs with mammary neoplasms are scarce. The present study aims to present epidemiological, clinical-pathological and overall survival data to help establish the prognosis and understand the biological behavior of CMNs. A total of 1539 cases were included (85% malignant and 13% benign). Tumor size was an important prognostic factor and was associated with overall patient survival (P< 0.0001). Most dogs diagnosed with malignant neoplasms (83%) had initial clinical staging, although 17% had regional or distant metastases at the time of diagnosis and lower overall survival (P<...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Female dogs; Mammary gland; Carcinomas; Prognosis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601714
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First description of basaloid carcinoma of the canine mammary gland: case report Arq. Bras. Med. Vet. Zootec.
Nakagaki,K.Y.R.; Gonçalves,A.B.B.; Rocha,R.M.; Cassali,G.D..
ABSTRACT The objective of this case report was to describe histopathological and immunohistochemical characteristics of the first reported basaloid carcinomas in the canine mammary gland. Two bitches were treated for tumors in the mammary gland and underwent mastectomy. Microscopic evaluation of these tumors revealed epithelial cells arranged in a predominantly solid pattern with hyperchromatic peripheral cells arranged in a palisade pattern. Metastases in regional lymph nodes were found in both animals, and one bitch exhibited pulmonary metastasis. Immunohistochemistry revealed positive labeling for the basal cell markers cytokeratin 14 and p63. Histopathological and immunohistochemical findings led to diagnoses of basaloid carcinoma of the canine mammary...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mammary gland; Salivary gland; Basaloid carcinoma; Basaloid adenoma; Solid carcinoma.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300878
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Glycogen-rich clear cell carcinoma of the feline mammary gland: case report Arq. Bras. Med. Vet. Zootec.
Campos,C.B.; Gamba,C.O.; Damasceno,K.A.; Lavalle,G.E.; Cassali,G.D..
ABSTRACT The aim of this report is to describe the first histopathological, immunohistochemical, and clinical characteristics of a feline glycogen-rich clear cell carcinoma (GRCCC). A Persian queen was admitted with mammary gland tumors and underwent radical unilateral mastectomy. Overall survival was considered 33 days and death was due to clinical evolution of the disease. Microscopic evaluation demonstrated epithelial cells arranged in a predominantly solid pattern, tumor cells presented an ample, granular, and foamy clear cytoplasm, and moderate cellular pleomorfism. The presence of cytoplasmatic glycogen was confirmed through diastase digestion followed by PAS staining. Histopathological and histochemical findings lead to the diagnosis of GRCCC with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Feline; Mammary gland; Neoplasm; Glycogen-rich clear cell carcinoma.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501117
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Increase in biofilm formation by Escherichia coli under conditions that mimic the mastitic mammary gland Ciência Rural
Costa,João Carlos Miguel; Espeschit,Isis de Freitas; Pieri,Fábio Alessandro; Benjamin,Laércio Anjos; Moreira,Maria Aparecida Scatamburlo.
Bacterial biofilms are involved in the aggravation and recurrence of clinical mastitis in dairy herds. Several factors such as pH, temperature, concentration of O2 and glucose can affect their induction and growth rates. In this study, biofilm production was demonstrated by 27 Escherichia coli strains isolated from bovine mastitis at different pH values depending on the availability of glucose, mimicking conditions found in mammary glands affected by the disease. Biofilm formation was analyzed by spectrophotometric analysis in microtiter plate with 16 different culture media and by scanning electron microscopy. Biofilm formation was greater in isolates cultured under conditions associated with low glucose availability (0.5% or 1.5%) and with either an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mammary gland; Escherichia coli; Milk; Acidity; Sugar.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000400015
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Invasive micropapillary carcinoma of the dog mammary gland: a case report Arq. Bras. Med. Vet. Zootec.
Cassali,G.D.; Serakides,R.; Gärtner,F.; Schmitt,F.C..
This report describes the morphological and immunohistochemical findings of two cases of breast invasive micropapillary carcinoma occurring in dogs. Histologically, the tumors are characterized by the presence of numerous irregular cystic formations filled out with nests of epithelial cells that exhibit a micropapillary pattern. These morphological features are characteristic of invasive micropapillary carcinoma in woman, a breast tumor not previously described in dogs.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Mammary carcinoma; Mammary gland; Invasive micropapillary carcinoma.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000400006
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Lipid peroxidation in female dogs bearing mammary gland carcinomas Arq. Bras. Med. Vet. Zootec.
Souza,I.B.; Cardoso,C.V.; Poppe,S.C.; Bondan,E.F..
RESUMO O estresse oxidativo causa peroxidação lipídica e formação de substâncias reativas ao ácido tiobarbitúrico (TBARS), processo que está comprovadamente associado à progressão de neoplasias malignas em seres humanos. Por sua vez, espécies reativas de oxigênio (EROs) são produzidas no processo carcinogênico, de forma que a geração de EROs parece ser, ao mesmo tempo, causa e consequência dele. Em cães, a associação da peroxidação lipídica com a carcinogênese permanece ainda obscura, com estudos escassos, de resultados conflitantes, que, muitas vezes, incluem, dentro de um mesmo grupo amostral, animais portadores de tumores heterogêneos dos pontos de vista morfológico e comportamental, além de estes se apresentarem em estágios bastante distintos. Nesse...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Dogs; Cancer; Mammary gland; Oxidative stress; TBARS..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501335
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Mammary botryomycosis: two concomitant cases in mares: case report Arq. Bras. Med. Vet. Zootec.
Belli,C.B.; Fonte,J.S.; Torres,L.N.; Gondin,M.R.; Maiorka,P.C.; Fernandes,C.B..
ABSTRACT Mammary disorders in mares are rare and usually only one animal per paddock is affected. In this report, two mares with 7 and 9 years old, were concomitantly diagnosis of chronic pyogranulomatous mastitis, associated with the Splendore-Hoeppli reaction, indicative of botryomycosis a rare, chronic suppurative disease with microabscess formation, characterized by in vivo formation of eosinophilic materials around microorganisms or biologically inert material. Various bacteria can cause botryomycosis in horses, and the most frequently isolated one is Staphylococcus sp., particularly S. aureus. This report confirms the role of Staphylococcus sp.; however, specifically S. hyicus and S. cohnii that prior to the current report, had not been associated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Equine; Mastitis; Mammary gland; Botryomycosis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000200342
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Mammary carcinosarcoma in cat: a case report Arq. Bras. Med. Vet. Zootec.
Paniago,J.D.G.; Vieira,A.L.S.; Ocarino,N.M.; França,S.A.; Malm,C.; Cassali,G.D.; Serakides,R..
A case of mammary carcinosarcoma is reported in a 13-year-old, mixed breed female cat, which was not spayed and had not received contraceptives. The patient presented extensive and coalescent nodules in all mammary glands. Based on the histological and immunohistochemical findings, the diagnosis of mammary carcinosarcoma was confirmed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cat; Carcinosarcoma; Mammary gland.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400008
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Mast cells and angiogenesis in canine mammary tumor Arq. Bras. Med. Vet. Zootec.
Lavalle,G.E; Bertagnolli,A.C; Tavares,W.L.F; Ferreira,M.A.N.D; Cassali,G.D.
The correlation between microvessel density and mast cells density in canine mammary tumors was studied. Sixty-five samples of canine mammary tumors, being 24 benign and 41 malignant, were analyzed. The routine Toluidine Blue staining method was used to assess the mast cells. To evaluate angiogenesis, the immunohistochemical expression of CD31 was assessed. There was no significant difference in either mast cells (P=0.44) or microvessel density (P=0.77) between malignant and benign tumors. A positive correlation was observed between microvessel density and mast cells (r=0.39; P=0.011) in malignant tumors. These results suggest that mast cells may play a role in canine mammary malignant tumors development, promoting angiogenesis, similar to some tumors...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bitch; Mammary gland; Mast cells; Microvessel density; Neoplasms.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600008
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Milk flow, teat morphology and subclinical mastitis prevalence in Gir cows PAB
Porcionato,Marco Aurélio de Felicio; Soares,Weber Vilas Boas; Reis,Carolina Barbosa Malek dos; Cortinhas,Cristina Simões; Mestieri,Lucinéia; Santos,Marcos Veiga dos.
The aim of this work was to evaluate the association between milk flow, teat morphological measurements and subclinical mastitis prevalence in Gir cows. Eighty cows in the 2nd and 3rd lactations, with 90 to 200 days of lactation, were divided according to milk flow during milking into fast or slow groups. Teat morphometry was assessed by ultrasound scanning of the right anterior teat and external measurements. Milk samples were collected for somatic cells count (SCC) and microbiological culture. The effect of milk flow during milking was evaluated by analysis of variance of milk yield, SCC, morphometry and external measurements. The association of morphometry and external measurements of the teats with the SCC and microorganisms found in milk were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gir; Lactation; Mammary gland; Milking; Morphology.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001200023
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Morphological changes induced by testosterone in the mammary glands of female Wistar rats BJMBR
Chambô-Filho,A.; Camargos,A.F.; Pereira,F.E.L..
Increased levels of androgens in postmenopausal women are considered to be a risk factor for breast cancer. Testosterone, alone or in combination with estrogen, induces epithelial dysplasia and mammary tumors in Noble rats. Since this model of hormone-induced neoplasia has not been reported in other rat strains, we studied the effect of testosterone on the mammary gland morphology of female Wistar rats. Sixty adult, non-castrated, female Wistar rats were implanted in the dorsum midline with a silicone tube containing 50 mg testosterone (testosterone propionate in 30 animals and non-esterified testosterone in the remaining 30 animals) and 20 additional animals were implanted with empty tubes and used as control. Five animals per group were killed 30, 60,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Testosterone; Androgens; Mammary gland; Mammary gland hyperplasia; Mammary gland tumors.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400008
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Pleomorphic lobular carcinoma of the canine mammary gland: histopathologic and immunohistochemical features Arq. Bras. Med. Vet. Zootec.
Cassali,G.D.; Gärtner,F.; Schmitt,F.C..
This report describes the morphological and immunohistochemical findings of a case of mammary gland pleomorphic lobular carcinoma occurring in the canine species. Histologically, it was characterized by the presence of tumor cells loosely dispersed in the stroma or arranged in a linear pattern showing relatively abundant eosinophilic cytoplasm with an eccentric nuclei. These morphological features, characteristic of pleomorphic mammary gland lobular carcinoma in woman, were not previously described in dogs.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Mammary carcinoma; Mammary gland; Pleomorphic lobular carcinoma.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000600006
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Prevalence and etiology of buffalo mastitis and milk somatic cell count in dry and rainy seasons in a buffalo herd from Analândia, São Paulo State, Brazil Arq. Bras. Med. Vet. Zootec.
Pizauro,L.J.L.; Silva,D.G.; Santana,A.M.; Clemente,V.; Lara,G.H.B.; Listoni,F.J.P.; Vaz,A.C.N.; Vidal-Martins,A.M.C.; Ribeiro,M.G.; Fagliari,J.J..
The aim of this study was to evaluate somatic cell count (SCC), prevalence and etiology of mastitis in a dairy buffalo herd from Analândia, São Paulo State, Brazil, in the dry and rainy seasons. Additionally, antimicrobial susceptibility profile of microorganisms isolated from milk samples was also evaluated. 1,042 milk samples from female Murrah buffaloes in a dairy farm located in Analândia, São Paulo State, Brazil, collected between May 2011 and November 2012 were analyzed. After the mammary gland physical examination, strip cup test and California Mastitis Test (CMT) were performed. Afterwards, 50mL of milk samples from each mammary quarter were collected aseptically for SCC in automatic equipment and microbiological examination. The antimicrobial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibiogram; Bubalus bubalis; SCC; Mammary gland; Microorganisms.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601703
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Produção de biofilmes por Staphylococcus spp. em vacas com mastite tratadas com homeopatia. Infoteca-e
ZAFALON, L. F.; CUNHA, M. L. R. S.; ALVES, T. C.; FERREIRA, E. M..
No Brasil, a homeopatia é a principal forma de controle da mastite em rebanhos orgânicos. Entretanto, desconhece-se a capacidade desse tipo de tratamento de controlar bactérias patogênicas relevantes à saúde animal e humana, como aquelas do gênero Staphylococcus. Esses micro-organismos apresentam fatores de patogenicidade, como a produção de biofilmes e questiona-se a possibilidade de essas bactérias permanecerem na glândula mamária de vacas tratadas com homeopatia, mesmo após o uso destas terapias alternativas. Assim, buscou-se investigar Staphylococcus spp. em leite de vacas com tratamento homeopático contra a mastite e a formação in vitro de biofilmes por esses patógenos. As vacas lactantes foram divididas em dois grupos antes do início do experimento,...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Mammary gland; Qualidade do leite; Sanidade Animal; Glândula Mamaria; Milk quality; Animal health.
Ano: 2019 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1110081
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Protocol for the anatomopathological examination of canine mammary tumors Arq. Bras. Med. Vet. Zootec.
Ferreira,E.; Bregunci,G.C.; Schmitt,F.C.; Cassali,G.D..
Foi elaborado um protocolo para exame anatomopatológico de tumores de mama em cães, constituído de três partes: requisição, exame clínico e laudo histopatológico. O exame clínico contém dados sobre a descrição macroscópica da lesão. O laudo histopatológico constituiu-se de campos para descrição microscópica pormenorizada das lesões e classificação da principal lesão observada. A elaboração do protocolo tem como objetivo estabelecer critérios para estudos e pesquisas sobre neoplasias mamárias em animais e auxiliar no diagnóstico e prognóstico de lesões mamárias.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neoplasia; Mammary gland; Histopathology; Protocol; Dog.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000100017
Registros recuperados: 24
Primeira ... 12 ... Última
 

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